Part:BBa_K4467002
PstSCR1 (from Puccinia striiformis var. striiformis)
Profile
Gene Name: Pst_12806
Subcellular localisation: Secreted protein
Length (bp): 444
Amino acids length: 146
Organism of origin: Puccinia striiformis var. striiformis
Properties and Biology
BBa_K4467002 is a protein coding sequence comprising the small fungal effector protein, Pst_12806, present in the haustorium-of the wheat stripe rust fungus, Puccinia striiformis f.sp.tritici (Pst). Upon infection, it is translocated into chloroplast and affects chloroplast function (Xu,Q., 2019). It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes (Xu,Q., 2019). Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS (Xu,Q., 2019).
Its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein.
Usage
As part of the 2022 UNSW_Australia project to design a peptide based inhibitor for Puccinia sp., we used Pst_12806 as a potential target. This protein was expressed in NEB TURBO and NEB T7 express E.coli cells. Its binding with TaiSP partner protein was analysed using FRET based interaction analysis.
References
XU, Q., et al. (2019). "An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function." Nature Communications 10(1): 5571.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 333
- 1000COMPATIBLE WITH RFC[1000]
| None |